At the Nateby Inn in Nateby, Cumbria, Gaynor Walker hopes her immaculate rooms and northern charm will impress her guests.
The B&Bers then head to Outfields Farm in Bedfordshire where Graham and Julia Ashley have a surprise for their fellow competitors.
The third visit is to At Home B&B in Chiswick, west London where artist Bob Osborne's irreverent attitude and casual approach to cleanliness causes confusion.
The last stop is to the Ammonite Lodge Guest House in Chard, Somerset.
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