Eighteen countries participated in the second semi-final. Russia was originally planned to perform in position three, but withdrew from the contest after the artist they selected was banned from entering Ukraine, resulting in countries originally planned to perform fourth and later, to do so one place earlier.
- Tijana Bogićević (Serbia) - "In Too Deep"
- Nathan Trent (Austria) - "Running on Air"
- Jana Burčeska (Macedonia) - "Dance Alone"
- Claudia Faniello (Malta) - "Breathlessly"
- Ilinca and Alex Florea (Romania) - "Yodel It!"
- O'G3NE (Netherlands) - "Lights and Shadows"
- Joci Pápai (Hungary) - "Origo"
- Anja (Denmark) - "Where I Am"
- Brendan Murray (Ireland) - "Dying to Try"
- Valentina Monetta and Jimmie Wilson (San Marino) - "Spirit of the Night"
- Jacques Houdek (Croatia) - "My Friend"
- JOWST5 (Norway) - "Grab the Moment"
- Timebelle (Switzerland) - "Apollo"
- Naviband (Belarus) - "Story of My Life"
- Kristian Kostov (Bulgaria) - "Beautiful Mess"
- Fusedmarc (Lithuania) - "Rain of Revolution"
- Koit Toome and Laura (Estonia) - "Verona"
- IMRI (Israel) - "I Feel Alive"
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